Quantification of diatoms in biofilms: Standardisation of methods

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dc.contributor.author Patil, J.S.
dc.contributor.author Anil, A.C.
dc.date.accessioned 2008-02-22T06:23:49Z
dc.date.available 2008-02-22T06:23:49Z
dc.date.issued 2005
dc.identifier.citation Biofouling, Vol.21; 181-188p.
dc.identifier.uri http://drs.nio.org/drs/handle/2264/1008
dc.description.abstract Benthic diatoms, which often dominate marine biofilms are mostly pennate along with a few centric species that have an attached mode of life. Even though the range of diatoms in biofilms is diverse, their ecology is poorly understood because of the difficulty in sampling and enumeration. Scraping or brushing are the traditional methods used for removal of diatoms from biofilms developed on solid substrata. The method of removal is the most critical step in enumerating the biofilm diatom community structure. In this study, a nylon brush and ceramic scraper were used as tools for the removal of diatoms from 1-4-day-old biofilms developed on fibreglass coupons and glass microscope slides. Standardisation of methods showed that the sample volume used in the analyses had the least influence on the quantification, whereas the method of removal was critical. The nylon brush was more efficient at recovering diatoms compared to a ceramic scraper. Direct microscopic enumeration of the community in the case of glass slides indicated that scraping resulted in between 30-50% underestimation. Heterogeneity in diatom community structure between replicate samples is one possible reason for such underestimation.
dc.language.iso en
dc.publisher Taylor and Francis
dc.rights Copyright [2005]. It is tried to respect the rights of the copyright holders to the best of the knowledge. If it is brought to our notice by copyright holder that the rights are voilated then the item would be withdrawn.
dc.subject biofilms
dc.subject substrata
dc.subject benthic environment
dc.subject colonization
dc.subject biofouling
dc.subject fouling organisms
dc.subject standardization
dc.subject technology
dc.subject methodology
dc.title Quantification of diatoms in biofilms: Standardisation of methods
dc.type Journal Article


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