Genotoxicity of cadmium in marine diatom Chaetoceros tenuissimus using the alkaline Comet assay

Show simple item record Desai, S.R. Verlecar, X.N. Nagarajappa Goswami, U. 2006-06-19T11:14:15Z 2006-06-19T11:14:15Z 2006
dc.identifier.citation Ecotoxicology, vol.15(4), 359-363p.
dc.description.abstract Genotoxic effects of Cadmium on phytoplankton Chaetoceros tenuissimus have been evaluated using DNA damage by comet assay. Cadmium concentrations ranging from 2.4 to 10 mg/L were used to evaluate the effects. Results showed that as the concentration of Cd increased growth of the diatom decreased. Alkaline single-cell gel electrophoresis (Comet assay) method, which is highly sensitive in detection of DNA damage in eukaryotic cells, was used to observe genomic changes in marine diatom cells. DNA damage was measured as percent number of comets and normal cells. 65% cells were found to be damaged at 10 mg/L concentration of Cd as compared to 23% in 2.4 mg/L and only 5% in controls. More than 50% apoptic cells were observed on 8th day at 10mg/L and 12th day at 7.5 mg/L concentrations. At lower Cd concentrations (4.5 mg/L and below) the damage was below 30% till the last day. This suggested that higher Cd levels have early damaging effects on cell nuclear material and that % injury increases with advancement of exposure period. One advantage of use of C. tenuissimus is the ease with which it can be cultured in a defined medium. Chaetoceros tenuissimus diatom can be used as an in vivo model for ecogenotoxicity assessment using the Comet assay.
dc.format.extent 78655 bytes
dc.format.mimetype application/pdf
dc.language.iso en
dc.publisher Springer
dc.rights An edited version of this paper was published by Springer. Copyright [2006] Springer
dc.subject Chaetoceros tenuissimus
dc.subject Comet assay
dc.subject Single-cell gel electrophoresis
dc.subject DNA damage
dc.title Genotoxicity of cadmium in marine diatom Chaetoceros tenuissimus using the alkaline Comet assay
dc.type Journal Article

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