DRS

DRS at National Institute Of Oceanography >
Institute's contributions >
Materials Sciences >

Please use this identifier to cite or link to this item: http://hdl.handle.net/2264/1321

Title: Role of beta 1-4 linked polymers in the biofilm structure of marine Pseudomonas sp. CE-2 on 304 stainless steel coupons
Authors: Jain, A.
Bhosle, N.B.
Citation: Biofouling, vol.24(4); 283-290p.
Issue Date: 2008
Publisher: Taylor and Francis
Abstract: Pseudomonas sp CE-2 cells attach and form biofilms on 304-stainless steel (SS) coupons. A series of experiments were carried out in order to understand the role of exopolysaccharides (EPS) in the formation and maintenance of CE-2 biofilms on SS coupons. The biofilm density and EPS concentration increased over the period of incubation and the highest values for both were recorded after 72 h. Calcofluor and the lectin concanavalin A (Con A) showed a positive interaction with 72-h old biofilms, indicating the presence of beta 1-4 linked polymers, and alpha-D-glucose and alpha-D-mannose in the biofilm matrix of CE-2. When the CE-2 cells were grown in the presence of calcofluor (200 mu g ml sup(-1)), biofilm formation was significantly reduced (approx. 85%). Conversely, the lectins Con A or WGA did not influence the CE-2 biofilms on the SS coupons. Furthermore, treatment with cellulase, an enzyme specific for the degradation of beta 1-4 linked polymers, removed substantial amounts of CE-2 biofilm from SS coupons. These results strongly suggest the involvement of beta 1-4 linked polymers in the formation and maintenance of Pseudomonas sp. CE-2 biofilms on SS coupons.
Document type: Journal Article
Copyright: This is a preprint of an article whose final and definitive form has been published in the "Biofouling" © 2008 Taylor & Francis; "Biofouling" is available online at http://www.informaworld.com/ with open URL of artilce : http://www.informaworld.com/openurl?genre=article&issn=0892-7014&volume=24&spage=183
URI: http://hdl.handle.net/2264/1321
Appears in Collections:Materials Sciences

Files in This Item:

File Description SizeFormat
Biofouling_24_283o.pdfOpen Access549.57 kBAdobe PDFView/Open

Items in DRS are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

DRS is a service of NIO library (NICMAS) | | Feedback: satya at nio.org

DSpace Software Copyright © 2002-2007 MIT and Hewlett-Packard | | Compliant to OAI-PMH V 2.0