Opisthorchis viverrini: Detection by polymerase chain reaction (PCR) in human stool samples
View/ Open
Date
2008Author
Umesha, K.R.
SanathKumar
Parvathi, A.
Duenngai, K.
Sithithaworn, P.
Karunasagar, Indrani
Karunasagar, Iddya
Metadata
Show full item recordAbstract
A polymerase chain reaction (PCR) assay was evaluated for detection of Opisthorchis viverrini eggs in the stool specimens of light and heavily infected individuals in Khon Kaen province of Thailand. A total of 75 fecal specimens were analyzed by PCR following DNA extraction. All the microscopically positive samples were positive by PCR, while 23 of 30 (76.6%) microscopically negative samples were also PCR positive. The sensitivity of the assay was 5 eggs/g of stool. This method is potentially useful in the diagnosis of human opisthorchiasis in endemic areas for treatment and in epidemiological investigations.
Collections
- Scholarly literature [8843]