Biochemical and molecular characterizaion of Bacillus pumilus isolated from coastal environment in Cochin, India

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dc.contributor.author Parvathi, A.
dc.contributor.author Krishna, K.
dc.contributor.author Jose, J.
dc.contributor.author Joseph, N.
dc.contributor.author Nair, S.
dc.date.accessioned 2009-06-23T11:18:30Z
dc.date.available 2009-06-23T11:18:30Z
dc.date.issued 2009
dc.identifier.citation Brazilian Journal of Microbiology, vol.40; 269-275
dc.identifier.uri http://drs.nio.org/drs/handle/2264/3364
dc.description.abstract Bacillus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. In this study, Bacillus strains isolated from the coastal environment of Cochin, India were identified by detailed conventional biochemical methods, fatty acid methyl ester (FAME) analysis and partial 16S rDNA sequencing. Analysis of the data revealed that Bacillus pumilus was the most predominant species in the region under study followed by B. cereus and B. sphaericus. The B. pumilus isolates were further characterized by arbitrarily primed PCR (AP-PCR), antibiotic sensitivity profiling and PCR screening for known toxin genes associated with Bacillus spp. All B. pumilus isolates were biochemically identical, exhibited high protease and lipase activity and uniformly sensitive to antibiotics tested in this study. One strain of B. pumilus harboured cereulide synthetase gene cesB of B. cereus which was indistinguishable from rest of the isolates biochemically and by AP-PCR. This study reports, for the first time, the presence of the emetic toxin gene cesB in B. pumilus
dc.language.iso en
dc.publisher Sociedade Brasileira de Microbiologia
dc.rights Copyright [2009]. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
dc.subject Bacillus pumilus
dc.subject microorganisms
dc.subject polymerase chain reaction
dc.subject biochemical analysis
dc.subject molecular structure
dc.subject coastal environment
dc.title Biochemical and molecular characterizaion of Bacillus pumilus isolated from coastal environment in Cochin, India
dc.type Journal Article


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