Denaturing gradient gel electrophoresis profiling of bacterial communities composition in Arabian Sea

Show simple item record Singh, SanjayKumar Ramaiah, N. 2011-06-15T12:07:21Z 2011-06-15T12:07:21Z 2011
dc.identifier.citation Journal of Environmental Biology,vol.32(3); 2011; 339-346
dc.description.abstract Denaturing gradient gel electrophoresis (DGGE) was used to elucidate spatial and temporal variations in bacterial community composition (BCC) from four locations along the central west coast of India. DNA extracts from 36 water samples collected from surface, mid-depth (approx.10 m) and close to bottom (approx.20 m) during premonsoon, postmonsoon, monsoon were analyzed by PCR for amplifying variable region of 16S rRNA gene and subsequently through DGGE. Prominent bands were excised, cloned and sequenced indicated the preponderance of gammaproteobacteria, bacteroidetes and cyanobacteria. Non-metric dimensional scaling of the DGGE gels indicated that the spatial variations in BCC were prominent among the sampling locations. Temporal variations in the BCC appear to be influenced by monsoonal processes. The canonical correspondence analyses suggest that the concentration of chlorophyll a and nitrate are two important environmental factors for both spatial and temporal variations in BCC. Chlorophyll a seems to be impart a top-down control of BCC while nitrate, the bottom-up control. Our results also suggest that BCC can vary over a small geographic distance in highly dynamic, seasonally predisposed tropical coastal waters.
dc.language.iso en
dc.publisher Triveni Enterprises
dc.rights Copyright [2011] Triveni Enterprises. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
dc.subject Bacterial community
dc.subject DGGE
dc.subject monsoon
dc.subject gammaproteobacteria
dc.title Denaturing gradient gel electrophoresis profiling of bacterial communities composition in Arabian Sea
dc.type Journal Article

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