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dc.contributor.authorParvathi, A.
dc.contributor.authorMendez, D.
dc.contributor.authorAnto, C.
dc.date.accessioned2011-07-29T07:26:43Z
dc.date.available2011-07-29T07:26:43Z
dc.date.issued2011
dc.identifier.citationIndian Journal of Microbiology, vol.51(3); 2011; 332-337
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/3888
dc.description.abstractThe marine-estuarine bacterium Vibrio harveyi is an important pathogen of invertebrates, most significantly, the larvae of commercially important shrimp Penaeus monodon. In this study, we analyzed V. harveyi isolated from shrimp hatchery environments for understanding the distribution of putative virulence genes and antimicrobial drug resistance. The putative genes targeted for PCR detection included four reversible toxin (Rtx)/hemolysin genes, a gene encoding homologue of Vibrio cholerae zonula occludens toxin (Zot) and a hemolysin-coregulated protein gene (hcp) by polymerase chain reaction (PCR). Of the four putative reversible toxin genes, vhh-1 was detected in 31% of the isolates, vhh-2 in 46%, vhh-3 in 23% and vhh-4 was detected in 27% of the isolates. A zot-like sequence of bacteriophage f237 was present in 15%, while hcp sequence was detected in 48% of the isolates. The antimicrobial susceptibility test revealed resistance to several groups of antibiotics including Beta-lactams, cephalosporins, macrolides, quinolones, nitrofurantoin and tetracycline
dc.language.isoen
dc.publisherSpringer
dc.rightsAn edited version of this paper was published by Springer. This paper is for R & D purpose and Copyright [2011] Association of Microbiologists of India.
dc.subjectVibrio harveyi
dc.subjectAntibiotic resistance
dc.subjectGenotyping
dc.subjectreversible toxin
dc.titleDistribution of putative virulence genes and antimicrobial drug resistance in Vibrio harveyi
dc.typeJournal Article


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