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Please use this identifier to cite or link to this item: http://drs.nio.org/drs/handle/2264/4077

Title: Characterization of Bacteria in Ballast Water Using MALDI-TOF Mass Spectrometry
Authors: Emami, K.
Askari, V.
Ullrich, M.
Mohinudeen, K.
Anil, A.C.
Khandeparker, L.
Burgess, J.G.
Mesbahi, E.
Citation: PLoS One, vol.7(6); 2012; e38515, 14pp.
Issue Date: 2012
Publisher: Public Library of Science
Abstract: To evaluate a rapid and cost-effective method for monitoring bacteria in ballast water, several marine bacterial isolates were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Since International Maritime Organization (IMO) regulations are concerned with the unintended transportation of pathogenic bacteria through ballast water, emphasis was placed on detecting species of Vibrio, enterococci and coliforms. Seawater samples collected from the North Sea were incubated in steel ballast tanks and the presence of potentially harmful species of Pseudomonas was also investigated. At the genus-level, the identification of thirty six isolates using MALDI-TOF MS produced similar results to those obtained by 16S rRNA gene sequencing. No pathogenic species were detected either by 16S rRNA gene analysis or by MALDI-TOF MS except for the opportunistically pathogenic bacterium Pseudomonas aeruginosa. In addition, in house software that calculated the correlation coefficient values (CCV) of the mass spectral raw data and their variation was developed and used to allow the rapid and efficient identification of marine bacteria in ballast water for the first time.
Document type: Journal Article
Copyright: Copyright: 2012 Emami et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
URI: http://drs.nio.org/drs/handle/2264/4077
Appears in Collections:Biological Sciences

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