Identifying the related compounds using electrospray ionization tandem mass spectrometry: bromotyrosine alkaloids from marine sponge Psammaplysilla purpurea

Show simple item record

dc.contributor.author Tilvi, S.
dc.contributor.author DeSouza, L.
dc.date.accessioned 2012-09-03T04:59:35Z
dc.date.available 2012-09-03T04:59:35Z
dc.date.issued 2012
dc.identifier.citation European Journal of Mass Spectrometry, vol.18; 2012; 333-343
dc.identifier.uri http://drs.nio.org/drs/handle/2264/4139
dc.description.abstract We have investigated extracts of marine sponge Psammaplysilla purpurea during three collections from Mandapam (Tamil Nadu, India) and Okha (Gujarat, India) and indentified two new bromotyrosine alkaloids, purpurealidin I (7) and J (8) using electrospray ionization tandem mass spectrometry (ESI-MS/MS). This sponge has tremendous chemical diversity of bromotyrosine alkaloids. Here we have used the proteomics approach in identifying related bromotyrosine alkaloids based on the predicated mass fragmentation pattern. The focus is on the examination of detailed product ion spectra of six known compounds that allowed identification of new compounds based on its mass fragmentation pattern. The isotopic pattern of the peaks for protonated molecules indicated the number of bromine atoms present in the molecule. During MS/MS studies, the most prominent product ion peak is for the presence of side chain propane with either free NH sub(2) or NHMe or Nme sub(2). The cleavage at C-C bond between oxime-amide carbonyl and amide-phenoxy moiety also gave characteristic product ions. The ESI-MS spectra for all three collections show that the bromotyrosine metabolites vary during different season and also geographical location. Although, some common metabolites were observed during the three collections. Thus, ESI-MS/MS is a method of choice in identifying the related compounds.
dc.language.iso en
dc.publisher IM Publications
dc.rights An edited version of this paper was published by © 2012 IM Publications LLP. The author version is uploaded for Intranet user...All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
dc.subject sponges
dc.subject organic compounds
dc.subject drugs
dc.subject Psammaplysilla purpurea
dc.title Identifying the related compounds using electrospray ionization tandem mass spectrometry: bromotyrosine alkaloids from marine sponge Psammaplysilla purpurea
dc.type Journal Article


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DRS


Advanced Search

Browse

My Account