Roseovarius azorensis sp. nov., isolated from seawater at Espalamaca, Azores

Show simple item record Rajasabapathy, R. Mohandass, C. Dastager, S.G. Liu, Q. Khieu, T.-N. Son, C.K. Li, W.-J. Colaco, A. 2014-03-05T10:44:24Z 2014-03-05T10:44:24Z 2014
dc.identifier.citation Antonie van Leeuwenhoek, vol.105(3); 2014; 571-578
dc.description.abstract A Gram-negative, motile, non-spore forming, rod shaped aerobic bacterium, designated strain SSW084<sup>T</sup>, was isolated from a surface seawater sample collected at Espalamaca (38°33`N; 28°39`W), Azores. Growth was found to occur from 15 to 40 °C (optimum 30 °C), at pH 7.0–9.0 (optimum pH 7.0) and with 25–100 % seawater or 0.5–7.0 % NaCl in the presence of Mg2<sup>+</sup> and Ca2<sup>+</sup>; no growth was found with NaCl alone. Colonies on seawater nutrient agar were observed to be punctiform, white, convex, circular, smooth, and translucent. Strain SSW084<sup>T</sup> did not grow on Zobell marine agar and tryptic soy agar even when seawater supplemented. The major respiratory quinone was found to be Q-10 and the G + C content was determined to be 61.9 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain SSW084<sup>T</sup> belongs to the genus Roseovarius and that its closest neighbours are Roseovarius tolerans EL-172<sup>T</sup>), Roseovarius mucosus DFL-24<sup>T</sup> and Roseovarius lutimaris 112<sup>T</sup> with 95.7, 95.4 and 95.3 % sequence similarity respectively. The remaining species of Roseovarius showed <95 % similarity. The polar lipids of strain SSW084<sup>T</sup> were determined to be phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified lipid and one unidentified aminolipid. The major fatty acids identified were identified as C<sub>18:1</sub> ω7c (52.5 %) and C<sub>16:0</sub> (13.8 %). On the basis of phenotypic, molecular and chemotaxonomic characteristics, strain SSW084<sup>T</sup> is considered to represent a novel species of the genus Roseovarius, for which Roseovarius azorensis sp. nov is proposed. The type strain is SSW084<sup>T</sup> (=KCTC 32421<sup>T</sup> = MTCC 11812<sup>T</sup>)
dc.language.iso en
dc.publisher Springer
dc.rights An edited version of this paper was published by Springer. This paper is for R & D purpose and Copyright [2014] Springer.
dc.subject new species
dc.subject biological speciation
dc.subject genetic isolation
dc.subject phylogenetics
dc.subject aerobic bacteria
dc.subject fatty acids
dc.title Roseovarius azorensis sp. nov., isolated from seawater at Espalamaca, Azores
dc.type Journal Article

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