Exploring rhizosphere bacteria of Eichhornia crassipes for metal tolerance and biological activity

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dc.contributor.author PrabhaDevi
dc.contributor.author Gomez, S.
dc.contributor.author Ribeiro, M.
dc.contributor.author Deshpande, S.A.
dc.contributor.author Singh, K.S.
dc.contributor.author DeSouza, L.
dc.date.accessioned 2015-08-24T09:40:04Z
dc.date.available 2015-08-24T09:40:04Z
dc.date.issued 2015
dc.identifier.citation Proceedings of 5th Annual International Conference on Advances in Biotechnology (BIOTECH 2015), 13-15th March 2015. (Fifth Annual Int. Conf. Advances in Biotechnology (BIOTECH 2015); IIT Kanpur; India; 13-15th Mar 2015). ; 2015; 1-10
dc.identifier.uri http://drs.nio.org/drs/handle/2264/4751
dc.description.abstract With urbanization and rapid industrial progress, water bodies around the world are getting highly polluted with metals. Bacteria have evolved several mechanisms to adapt to heally metal contaminants thus representing a potential source for bioremediation process. The present study deals with isohtion, identifiation and characterization of metal tolerant bacteria(MTB) from the rhizosphere of Eichhornia crassipes (water hyacinth) collected from the backwaters of Cochin (South India, 9°58'N, 76°l7'E). Depending on the metals they tolreted (Pb, Cu, Fe, Zn. & Co), the metal tolerant bacteria were labeled as Pb-1 to Pb-3, Cu-1 to Cu-4, Fe-1 to Fe-4,Zn-1 to Zn-4, and Co-1 to Co-2. No cultures tolerated and grew in the presence of Hg and Cd. Cultures were identified on the basis of 16s rRNA gene sequence analysis. Morphological and compositional studies were conducted using computer-assisted Scanning Electron Microscopy equipped with Energy Dispersive Spectroscopy (SEM/EDS). The maximum tolerable concentration (MTC) which is the highest concentration having minimum effect on the growth of the isolates were determined and found to be <2mM for CuSO<sub>4</sub>, <3mM for ZnSO<sub>4</sub> <3.5mM for CoCl<sub>2</sub>, <12mM for PbNO<sub>3</sub> and 25mM for FeCl<sub>3</sub>, They were further screened for antibiotic sensitivity and biological activity according to Kirby-Bauer disc diffusion method The MTB under metal stress condition showed significant biological activity against clinical pathogens, fouling bacteria and selective plant and fish pathogens indicating their biotechnological application in medicine, agriculture and aquaculture.
dc.language.iso en
dc.publisher GSTF
dc.rights Copyright [2015]. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
dc.subject MICROBIOLOGY::General
dc.title Exploring rhizosphere bacteria of Eichhornia crassipes for metal tolerance and biological activity
dc.type Journal Article

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