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dc.contributor.authorPatil, J.S.
dc.contributor.authorSaino, T.
dc.date.accessioned2015-10-01T13:13:12Z
dc.date.available2015-10-01T13:13:12Z
dc.date.issued2015
dc.identifier.citationBiofouling, vol.31(5); 2015; 417-432
dc.identifier.otherno
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/4793
dc.description.abstractThis study evaluates, for the first time, the influence of biofilms on single and double optical window (SOW and DOW, respectively) fast repetition rate fluorometer (FRRF) measurements of microalgal photosystem-II initial fluorescence (F<sub>0</sub>), maximum fluorescence (F<sub>m</sub>), variable fluorescence (F<sub>v</sub>= F<sub>m</sub> – F<sub>0</sub>), quantum yield(F<sub>v</sub>/F<sub>m</sub>) and functional absorption cross section (σPSII)]. Biofilms with chlorophyll > 0.1 μg cm<sup>-2</sup> and > 0.3 μgcm<sup>-2</sup> on SOW and DOW, respectively, produced a substantial increase in fluorescence. However, the relative magnitude of biofouling effects depended on sample chlorophyll concentrations, being more critical at concentrations < 1 mg m<sup>-3</sup>. In DOW-FRRF, biofilms affected F<sub>0</sub> (increased) and F<sub>v</sub>/F<sub>m</sub>) (decreased) but not F<sub>v</sub> and σPSII, whereas in SOW-FRRF, biofilms increased fluorescence and showed a variable effect on F<sub>v</sub> F<sub>m</sub> and σPSII, because only biofilms on SOW attained actual F<sub>m</sub>. As a result, the biofilm effect was substantial on SOW-FRRF measurements. On the other hand, the neutral-density filters (representing non-chlorophyll containing biofilms) with different transmission levels reduced the fluorescence signal. Correction procedures for the above photosystem-II parameters are proposed here.
dc.language.isoen
dc.publisherTaylor and Francis
dc.relation.requiresBiofouling_31_417.jpg
dc.rightsThe final and definitive form of the preprint has been published in the "Biofouling" © 2015 Taylor & Francis; "Biofouling" is available online at http://www.tandfonline.com with open URL of article : http://dx.doi.org/10.1080/08927014.2015.1052416. The term & condition associated with this preprint is at http://www.tandfonline.com/page/terms-and-conditions
dc.subjectCHEMISTRY AND BIOGEOCHEMISTRY
dc.titleEffect of biofilm on fluorescence measurements derived from fast repetition rate fluorometers
dc.typeJournal Article


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