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dc.contributor.authorBhagat, J.
dc.contributor.authorIngole, B.S.
dc.date.accessioned2015-10-16T05:50:56Z
dc.date.available2015-10-16T05:50:56Z
dc.date.issued2015
dc.identifier.citationEnvironmental Science and Pollution Research, vol.22(14); 2015; 10758-10768
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/4795
dc.description.abstractIn vivo and in vitro exposures were used to investigate the genotoxicity of mercuric chloride (HgCl<sub>2</sub>) to the marine snail, Planaxis sulcatus. The comet assay protocol was validated on gill cells exposed in vitro to hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>, 0–50 μM). Snails were exposed in vivo for 96 h to HgCl<sub>2</sub> (10, 20, 50, and 100 μg/l). Our results showed significant concentration-dependent increase in the tail DNA (TDNA) and olive tail moment (OTM) in exposed snails for all doses compared with controls. In vitro exposure to HgCl<sub>2</sub>(10–100 μg/l) resulted in significantly higher values for TDNA at all concentrations. Our results showed that DNA damage increased in the gill cell with increasing exposure time. This study demonstrates the usefulness of comet assay for detection of DNA damage after exposure to HgCl<sub>2</sub> and the sensitivity of marine snail P. sulcatus as a good candidate species for metal pollution
dc.language.isoen
dc.publisherSpringer
dc.rightsAn edited version of this paper was published by Springer. This paper is for R & D purpose and Copyright [2015] Springer.
dc.subjectBIOLOGY::Physiology, biochemistry, biophysics
dc.subjectAQUATIC POLLUTION::Effects on organisms
dc.subjectMOLLUSCS::Physiology, biochemistry, biophysics
dc.titleGenotoxic potency of mercuric chloride in gill cells of marine gastropod Planaxis sulcatus using comet assay
dc.typeJournal Article


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