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dc.contributor.authorGupta, Vasundhera
dc.contributor.authorSingh, P.K.
dc.contributor.authorKorpole, S.
dc.contributor.authorSrinivas, T.N.R.
dc.contributor.authorAnilKumar, P.
dc.date.accessioned2018-06-06
dc.date.accessioned2018-10-23T09:24:57Z
dc.date.available2017-07-01
dc.date.available2018-10-23T09:24:57Z
dc.date.issued2017
dc.identifier.citationInternational Journal of Systematic and Evolutionary Microbiology, vol.67(7); 2017; 2219-2224
dc.identifier.issn1466-5026
dc.identifier.urihttps://doi.org/10.1099/ijsem.0.001928
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/7833
dc.description.abstractA facultatively anaerobic, endospore forming, alkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK61T, was isolated from a sediment sample collected from Coringa mangrove forest, India. Colonies were circular, 1.5?mm in diameter, shiny, smooth, yellowish and convex with entire margins after 48?h growth at 30?�C. Growth occurred at 15-42?�C, with 0-3?% (w/v) NaCl and at pH 6-9. AK61T was positive for amylase activity and negative for oxidase, catalase, aesculinase, caseinase, cellulase, DNase, gelatinase, lipase and urease activities. The fatty acids were dominated by branched types with iso- and anteiso- saturated fatty acids with a high abundance of iso-C14?:?0, iso-C15?:?0, anteiso-C15?:?0 and iso-C16?:?0; the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid; and MK-7 was the major menaquinone. DNA-DNA hybridization between AK61T and� Bacillus indicus MTCC 4374T and between AK61T and� Bacillus indicus KCTC 3880 showed relatedness of 37.99 and 33.32?%?respectively. The DNA G+C content of AK61T was 44?mol%. The results of a blast sequence similarity search based on 16S rRNA gene sequences indicated that� Bacillus cibi and� Bacillus indicus were the nearest phylogenetic neighbours, with a pair-wise sequence similarity of 97.69 and 97.55?%?respectively. The results of phylogenetic analysis indicated that AK61T was clustered with� Bacillus idriensis and� Bacillus indicus . On the basis of its phenotypic characteristics and phylogenetic inference, AK61T represents a novel species of the genus� Bacillus , for which the name Bacillus mangrovi sp. nov. is proposed. The type strain is AK61T (=JCM 31087T=MTCC 12015T=KCTC 33872T).
dc.language.isoen
dc.publisherMicrobiology Society
dc.relation.ispartofInt J Syst Evol Microbiol
dc.relation.isreferencedbySCI
dc.titleBacillus mangrovi sp. nov., isolated from a sediment sample from a mangrove forest
dc.typeJournal Article
dc.affiliation.authorMTCC-Microbial Type Culture Collection & Gene Bank, CSIR-Institute of Microbial Technology, Chandigarh-160036, India


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