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dc.contributor.authorMitbavkar, S.
dc.contributor.authorAnil, A.C.
dc.date.accessioned2008-02-12T04:35:03Z
dc.date.available2008-02-12T04:35:03Z
dc.date.issued2006
dc.identifier.citationCryobiology, Vol.53; 143-147p.
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/815
dc.description.abstractTwo pennate microphytobenthic diatoms, Amphora coffeaeformis (Agardh) Kutzing and Navicula transitans var. derasa f. delicatula Heimdal, were cryopreserved and monitored on thawing to track the mechanical injuries and their post-preservation recovery. Cells were subjected to (1) direct freezing in liquid nitrogen and (2) two-step cooling with and without the cryoprotectant, dimethyl sulfoxide (Me2 SO). Mechanical injury due to exposure to low temperature diVered between the two species. While A. coffeaeformis cells were intact and could survive even direct freezing without a cryoprotectant, N. delicatula cell chloroplasts were damaged. However, the two-step cooling along with a cryoprotectant minimized the mechanical injury to cells of both species thereby enhancing the post-thaw viability.
dc.language.isoen
dc.publisherElsevier
dc.rightsCopyright [2006]. It is tried to respect the rights of the copyright holders to the best of the knowledge. If it is brought to our notice by copyright holder that the rights are voilated then the item would be withdrawn.
dc.subjectphytoplankton culture
dc.subjectcell culture
dc.subjectfreezing storage
dc.subjectcryobiology
dc.subjectbilogical damage
dc.subjectintertidal environment
dc.subjectAmphora coffeaeformis
dc.subjectNavicula delicatula
dc.titleCell damage and recovery in cryopreserved microphytobenthic diatoms
dc.typeJournal Article


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