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dc.contributor.authorSingh, SanjayKumar
dc.contributor.authorRamaiah, N.
dc.date.accessioned2011-06-15T12:07:21Z
dc.date.available2011-06-15T12:07:21Z
dc.date.issued2011
dc.identifier.citationJournal of Environmental Biology,vol.32(3); 2011; 339-346
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/3856
dc.description.abstractDenaturing gradient gel electrophoresis (DGGE) was used to elucidate spatial and temporal variations in bacterial community composition (BCC) from four locations along the central west coast of India. DNA extracts from 36 water samples collected from surface, mid-depth (approx.10 m) and close to bottom (approx.20 m) during premonsoon, postmonsoon, monsoon were analyzed by PCR for amplifying variable region of 16S rRNA gene and subsequently through DGGE. Prominent bands were excised, cloned and sequenced indicated the preponderance of gammaproteobacteria, bacteroidetes and cyanobacteria. Non-metric dimensional scaling of the DGGE gels indicated that the spatial variations in BCC were prominent among the sampling locations. Temporal variations in the BCC appear to be influenced by monsoonal processes. The canonical correspondence analyses suggest that the concentration of chlorophyll a and nitrate are two important environmental factors for both spatial and temporal variations in BCC. Chlorophyll a seems to be impart a top-down control of BCC while nitrate, the bottom-up control. Our results also suggest that BCC can vary over a small geographic distance in highly dynamic, seasonally predisposed tropical coastal waters.
dc.language.isoen
dc.publisherTriveni Enterprises
dc.rightsCopyright [2011] Triveni Enterprises. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
dc.subjectBacterial community
dc.subjectDGGE
dc.subjectmonsoon
dc.subjectgammaproteobacteria
dc.titleDenaturing gradient gel electrophoresis profiling of bacterial communities composition in Arabian Sea
dc.typeJournal Article


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