Imtechella halotolerans gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from estuarine water
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A novel Gram-negative, rod-shaped, non-motile, non-sporulating bacterium, designated strain K1 sup(T), was isolated from an estuarine water sample collected from Kochi, Kerala, India. Colonies on marine agar were circular, 2.0-2.5 mm in diameter, shiny, yellow, translucent and convex with entire margins. Strain K1 sup(T) was negative for ornithine decarboxylase, lysine decarboxylase, nitrate reduction and H sub(2)S production. The fatty acids were dominated by iso-branched components with a high abundance of iso-C sub(15:0), iso-C sub(15:1) G and iso-C sub(17:0) 3-OH; MK-6 (64 percent) and MK-7 (34 percent) were found as major respiratory quinones; and phosphatidylethanolamine, two unidentified aminolipids, four unidentified phospholipids and two unidentified lipids were major polar lipids. The DNA G+C content of strain K1 sup(T) was 46.1 molpercent. 16S rRNA gene sequence analysis indicated that strain K1 sup(T) was related most closely to the type strain of Zhouia amylolytica (pairwise sequence similarity of 93.0 percent). Phylogenetic analysis showed that strain K1 sup(T) formed a distinct branch within the family Flavobacteriaceae and clustered with the clade comprising species of the genera Zhouia, Coenonia and Capnocytophaga, being phylogenetically most closely related to the type strain of Zhouia amylolytica at a distance of 9.2percent (90.8 percent similarity). Other species of the genera within the same clade were related to strain K1 sup(T) at distances of 15.0-23.1 percent. Based on phenotypic and chemotaxonomic characteristics and on phylogenetic inference, strain K1 sup(T) is considered to represent a novel species of a new genus in the family Flavobacteriaceae, for which the name Imtechella halotolerans gen. nov., sp. nov. is proposed. The type strain of Imtechella halotolerans is K1 sup(T) (5MTCC 11055T5JCM 17677T).
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