Bacillus lacus sp. nov., isolated from a water sample of a salt lake in India
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A strictly aerobic, alkaliphilic, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK74-T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India. Colonies were circular, 1.2 mm in diameter, shiny, smooth, whitish and convex with an entire margin after 48 h growth at 37oC with pH 9.0. Growth occurred at 25-42oC, 0-4% (w/v) NaCl and at a pH of 7-12. Strain AK74-T was positive for aesculinase, caseinase, lipase activities and negative for oxidase, catalase, amylase, cellulase, DNase, gelatinase and urease activities. The fatty acids were dominated by branched iso-, anteiso- and saturated fatty acids with a high abundance of iso-C15:0, anteiso-C15:0, C16:0 and C16:1 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The DNA G+C?content of strain AK74-T was 51.6 mol%. A blast sequence similarity search based on 16S rRNA gene sequences indicated that Bacillus niabensis , Bacillus idriensis and Bacillus halosaccharovorans were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 96.6, 96.6 and 96.5%, respectively. Phylogenetic analysis showed that strain AK74-T clustered with Bacillus mangrove and together clustered with Bacillus idriensis and Bacillus indicus . Based on its phenotypic characteristics and on phylogenetic inference, strain AK74-T represents a novel species of the genus Bacillus , for which the name Bacillus lacus sp. nov. is proposed. The type strain is AK74T (=MTCC 12638-T=KCTC 33946-T=JCM 32185-T).
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